Acid-Fast Bacteria Stain โ TB & Mycobacteria
The Ziehl-Neelsen (ZN) stain is the gold standard method for detecting acid-fast bacteria (AFB), particularly Mycobacterium tuberculosis, in clinical specimens. This differential stain exploits the unique waxy cell walls of mycobacteria that contain mycolic acids, making them resistant to decolorization by acid-alcohol after staining with carbol fuchsin. The kit produces distinct red acid-fast bacilli against a blue background, allowing rapid screening for tuberculosis and other mycobacterial infections. Essential for microbiology laboratories, pathology departments, and TB diagnostic centers. The method can be applied to tissue sections, sputum smears, and other clinical specimens.
Stain bright red or pinkish-red. Rod-shaped bacteria may appear singly, in clusters, or in "schooling" patterns. Mycolic acid cell walls retain carbol fuchsin.
Nuclei and non-acid-fast bacteria stain blue with methylene blue counterstain. Provides clear contrast for AFB identification.
Weaker acid decolorization for demonstrating M. leprae in tissue sections and other less acid-fast organisms.
Essential for TB diagnosis, leprosy identification, and detecting atypical mycobacteria in immunocompromised patients.
Contact our team for specifications, protocols, and availability.